Thus,it plays an important role for the creation of new variation in crops. The second chapter deals with the literature review and the third chapter describes protocols used in these experiments. This book begins with a general introduction where more information was found as to the global status on rice production and its biotechnology. Such technologies improve the quality of breeding processes and enrich the existing germplasm. Chapter four and five concentrates the outcome of the work and general discussion respectively. I hope this book will give more information to an international audience including researchers, graduate and undergraduate students, teachers and professionals who are working in Botany, Agriculture and other related life sciences. It is hoped that improvements of cereal crops now possible through the application of plant cell and tissue culture.